In a "boy vs. girl" challenge, two YouTubers are in a room with 100 mystery buttons voiced by other popular YouTubers. Each button can trigger a punishment, reward, or trivia question. The loser of the challenge has to stay overnight in the room. Throughout the video, the two participants take turns pushing buttons, answering questions, and receiving various punishments, including being covered in paint, pudding, and other substances. In the end, the female participant successfully finds the "escape" button and wins the challenge, leaving the male participant to stay overnight in the room.
Here are the key facts extracted from the text:
1. There are 100 mystery buttons in the challenge.
2. The challenge is a boy versus girl competition.
3. Jenna Marbles is mentioned in the challenge.
4. The loser of the challenge has to stay in a box overnight by themselves.
5. The participants have to push buttons and complete tasks.
6. The tasks include trivia questions, punishments, and rewards.
7. One of the participants, Chase, is mentioned throughout the challenge.
8. Chase's husband's name is Preston.
9. The challenge includes a competition within a competition where the participants have to keep a donut on their head the longest.
10. The participants have to answer questions about their YouTube channel, such as the number of subscribers and the title of their most viewed video.
11. The challenge includes a task where the participants have to eat a mysterious substance.
12. The participants have to do a "smolder" at the camera and get rated by the audience.
13. There is a chance for viewers to win a signed computer by the participant.
14. The challenge includes a task where the participants have to escape a box.
15. The participant who wins the challenge gets to escape the box, while the loser has to stay overnight.
16. Chase has to stay the night in the box because he lost the challenge.