The summary of the text is:
The text is a transcript of a video where the speaker explains how he used DIY gene therapy to cure his lactose intolerance. He describes the science behind lactose, lactase, and the virus he used to deliver the gene for lactase to his intestinal cells. He shows how he prepared the virus, packaged it into a pill, and tested it by eating pizza. He claims that the therapy worked and that he can now enjoy dairy products without getting sick. He also mentions that this project is still in development and that he will update his viewers on his progress.
Here are some possible facts extracted from the text:
1. The speaker became lactose intolerant at the age of 15 and suffered from severe symptoms when consuming dairy products.
2. The speaker studied biology and developed a DIY gene therapy to restore their lactose tolerance using a virus that delivers the Lac Z gene to their intestinal cells.
3. The virus is called an AAV or adeno-associated virus, which is a common and safe virus that can integrate its DNA into specific locations in the host genome.
4. The speaker used three pieces of DNA to make the virus: one that contains the Lac Z gene flanked by ITR sequences, and two that contain the proteins that make up the virus shell.
5. The speaker used lipofectamine, a chemical that forms lipid bubbles around the DNA, to introduce the DNA into two types of mammalian cells: HEC and Cho cells.
6. The speaker harvested the virus from the media and the cells after three days and packaged it into a pill with microcrystalline cellulose.
7. The speaker tested the therapy by taking the pill and eating a cheesy pizza, and reported no symptoms of lactose intolerance after 48 hours.
8. The speaker used a color-changing dye called X-gal to confirm that the cells were producing lactase, the enzyme that breaks down lactose.